none 10.31254/phyto.2026.15206 BioMed Research Publishers BioMed Research Publishers 16195 194022388 316921 20260428131321781 10.31254 2026-04-28T13:13:29Z 2026-04-28T13:13:28Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 04 27 2026 15 2 Prerna Singh Department of Pharmacology, JN Medical College & Hospital, Aligarh Muslim University (AMU), Aligarh-202002, Uttar Pradesh, India https://orcid.org/0009-0003-2417-7871 Aijaz Abbas Rizvi Department of Physiology, JN Medical College & Hospital, Aligarh Muslim University (AMU), Aligarh-202002, Uttar Pradesh, India https://orcid.org/0009-0000-7646-466X Mehdi Hayat Shahi Interdisciplinary Brain Research Centre, JN Medical College & Hospital, Aligarh Muslim University (AMU), Aligarh-202002, Uttar Pradesh, India https://orcid.org/0000-0001-9589-4382 Shagufta Moin Department of Biochemistry, JN Medical College & Hospital, Aligarh Muslim University (AMU), Aligarh-202002, Uttar Pradesh, India https://orcid.org/0000-0003-2116-4832 Waseem Rizvi Department of Pharmacology, JN Medical College & Hospital, Aligarh Muslim University (AMU), Aligarh-202002, Uttar Pradesh, India https://orcid.org/0000-0003-0850-090X Syed Shariq Naeem Department of Pharmacology, J.N. Medical College, Aligarh Muslim University, Aligarh-202002, Uttar pradesh, India https://orcid.org/0000-0003-4460-4597 Background: Plant products are increasingly seen as promising alternatives to anticancer therapy due to their intrinsic safety and non-toxicity profiles. Objective: The main area of our research is searching for pharmacologically active compounds from a natural source. The present study aimed to screen the role of Carica papaya (C. papaya) leaf extract and Zamzam water on cadmium-induced brain tumours and oxidative stress in rat models. Material and Methods: Oxidative stress and cancer were induced by oral Cadmium for 60 days, followed by treatment with C. papaya leaf ethanolic extract at 200 and 400 mg/kg and Zamzam water 1 mL/Kg for 30 days. At the end of the study, the animals were sacrificed, and the antioxidant activity of extracts was assayed by in vivo antioxidant tests (CAT, MDA, GSH). To assess anti-tumour activity, expression of Bmi-1 and p53 was evaluated in brain tissue. Results: Ethanolic extract of leaves of C. papaya in the dose of 200 mg/kg and 400 mg/kg significantly decreased Cadmium-induced oxidative stress. At the same time, Zamzam water reduced the stress, but it was not significant. C. papaya in high dose (400 mg/kg) and Zamzam water reduced the expression of Bmi-1 while increasing the expression of p53 in both low and high doses (200 mg/kg and 400 mg/kg). Conclusion: Our findings suggest that C. papaya leaf extract and Zamzam water have anti-tumour potential. 04 27 2026 158 163 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2026.15206 https://phytopharmajournal.com/articles/abstracts/897/year=2026&vol=15&issue=2