none 10.31254/phyto.2026.15203 BioMed Research Publishers BioMed Research Publishers 16195 193583305 316921 20260420131520875 10.31254 2026-04-20T13:15:27Z 2026-04-20T13:15:26Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 04 17 2026 15 2 Kyadagi Ravikumara Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru-570006, Karnataka, India https://orcid.org/0009-0000-0772-8916 Taralabalu Matt Kaveri Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru-570006, Karnataka, India https://orcid.org/0000-0002-7954-7468 S. Umesha Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru-570006, Karnataka, India https://orcid.org/0000-0003-4851-1443 Background: Bignonia magnifica, known as glow vine or purple funnel vine, is a stunning evergreen climber from the Bignoniaceae family, native to humid forests in Colombia, Ecuador, Panama, and Venezuela. It has an aesthetic value and potential medicinal properties. Objectives: The present study was carried out to evaluate phytochemical compositions and antioxidant activity of B. magnifica leaf extracts. Materials and Methods: Sequential leaf extraction was performed by Soxhlet apparatus using Chloroform, Ethyl acetate, Ethanol and Methanol solvents. The qualitative and quantitatively estimation of phytochemical constituents by using standard procedure. High Resolution- Liquid Chromatography Mass Spectroscopy analysis to identify bioactive compounds. The antioxidant activity was evaluated by ferric reducing antioxidant power assay. Results: Preliminary phytochemical screening of leaf extracts revealed the presence of carbohydrates, tannins, phenols, flavonoids, terpenoids, proteins, alkaloids, saponins, glycosides, and cardiac glycosides. The Quantitative analysis indicates that the ethyl acetate solution has a high phenolic content (49.50±0.07 mg/g) and tannin (506.00±0.041TAE) content. In contrast, the chloroform infusion shows increased flavonoid levels (4.00±0.001mg/gQE), terpenoids (1.42±0.002mg-TPE/g) and alkaloids (16.50±0.58%). The FRAP assay measures the antioxidant task and demonstrates that ethyl acetate (2241.07±0.01mg/gAAE) infusion has a greater diminishing effect than chloroform (1062.50±0.01 mg/gAAE) infusion. The HR-LCMS analysis identified bioactive compounds such as Genistein, Hydroquinidine, 7',8'-Dihydro-8' hydroxycitraniaxanthin, 14,19- Dihydroaspidospermatine, Pyrrhoxanthinol, Notoginsenoside R10. Conclusion: These findings indicate that the B. magnifica leaf contain a significant amount of bioactive substances and natural antioxidants, which supports the idea that the leaf could be used as a curative agent in the treatment of oxidative stress disorders and medicinal properties. 04 17 2026 135 143 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2026.15203 https://phytopharmajournal.com/articles/abstracts/894/year=2026&vol=15&issue=2