none 10.31254/phyto.2026.15106 BioMed Research Publishers BioMed Research Publishers 16195 192875616 316921 20260407013135159 10.31254 2026-04-07T01:31:37Z 2026-04-07T01:31:36Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 03 30 2026 15 1 T Ahash https://orcid.org/0009-0004-7137-4226 A Jagadeeswaran https://orcid.org/0000-0002-7024-4794 Sakthi Priya M https://orcid.org/0000-0001-6425-1069 K Sukumar https://orcid.org/0000-0002-2621-2787 Background: In poultry, effective immunostimulation not only strengthens defenses against pathogens but also ensures timely resolution of inflammation, allowing nutrients to be redirected to growth and productivity. It helps to counteract immunosuppression caused by stress factors and environmental challenges, thereby maintaining bird health and productivity. Proper immunostimulation can also improve resistance to pathogens, ultimately leading to better overall flock performance. Objective: The present study aimed to find out the suitable ration of inclusive ingredients of Triphala powder, viz., Terminalia chebula, Terminalia bellerica and Emblica officinalis (in order) by assessing their influences on growth, immune responses and antioxidant status in layer cockerels. Materials and Methods: A total of 140 BV-300 cockerels were divided into seven groups: control group (fed with basal diet alone) and six treatment groups receiving Triphala powder with three different ratios of ingredients [1:1:1, 1:2:3 and 1:2:4 (in order of ingredients)] at two different (0.5% and 1% of basal diet) inclusion levels. Growth performance was assessed by calculating weekly body weight gains. Humoral immune responses were evaluated by haemagglutination inhibition (HI) titre against Newcastle disease antigen and haemagglutination (HA) titre against Sheep Red Blood Cells (SRBC). Cell mediated immune response was assessed by dinitrochlorobenzene (DNCB) - induced cutaneous delayed hypersensitivity test. Antioxidant status was judged by estimating antioxidant enzyme activities viz., superoxide dismutase (SOD) and catalase (CAT) activities, in the liver tissue. Results: Results revealed that supplementation with Triphala powder enhanced growth at all the three different ratios at both the levels of inclusion (0.5% and 1%), with significant effect noticed at 0.5% inclusion level of 1:2:3 ratio. This level also significantly enhanced both humoral and cell-mediated immune responses. Antioxidant enzyme activities were also markedly elevated in groups receiving Triphala powder, with significant effects observed in 1:2:4 ratio (1%) group. No morbidity or mortality were observed during the study period, indicated the inclusion levels or the ratio of ingredients were not obnoxious to the cockerels and proved the safety of the Triphala preparation in the cockerels. Conclusion: Triphala supplementation improved growth performance and immune responses at 0.5% inclusion level in 1:2:3 ratio, while 1% inclusion level in 1:2:4 ratio exhibited superior antioxidant potential, suggesting greater benefits under stress conditions such as heat stress or disease challenges. 03 30 2026 46 55 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2026.15106 https://phytopharmajournal.com/articles/abstracts/881/year=2026&vol=15&issue=1