none 10.31254/phyto.2025.14613 BioMed Research Publishers BioMed Research Publishers 16195 189926750 316921 20260210022520887 10.31254 2026-02-10T02:25:27Z 2026-02-10T02:25:26Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 1 30 2026 14 6 Blondo P Metsebing https://orcid.org/0009-0000-1434-7900 Romuald Oba https://orcid.org/0009-0005-5794-0022 Landry G Kengne https://orcid.org/0000-0002-0973-513X Nkodo Ekeme https://orcid.org/0009-0003-2548-1891 Regine G Mboubou https://orcid.org/0009-0002-4696-1432 Maximillienne A Nyegue https://orcid.org/0000-0002-9642-9930 Choupo Guifo https://orcid.org/0009-0004-4158-4070 Fabrice T Tsigain https://orcid.org/0009-0005-8536-4464 Rosemary T Kingue https://orcid.org/0000-0002-5402-1021 Astride C Djeuani https://orcid.org/0000-0002-4299-8759 Dominique C Mossebo Background: Ganoderma resinaceum is a parasitic fungus of oil palms and fruit trees in Africa in general and Cameroon in particular, where it often affects almost half of the plantation stands, causing destruction on several levels. Aims and Objectives: In order to solve the problems of trunk base rot commonly encountered in palm groves and fruit crop fields, which is responsible for enormous losses to human food supplies and the economy, Citrus sinensis essential oil was tested on Ganoderma resinaceum mycelium and its inhibitory and fungicidal activity was evaluated. Material and Methods: The mycelium of G. resinaceum is obtained from cultures on PDA medium with fragments of carpophores collected from the trunks of fruit trees. The essential oil of C. sinensis was extracted by hydrodistillation method using a Clevenger apparatus. This essential oil is tested on the mycelial growth of G. resinaceum by microatmosphere and direct contact method. Results: The yield of essential oil extracted from the pericarp of C. sinensis is 0.84%. This oil, slows the growth of G. resinaceum mycelium at concentrations of 0.125, 0.25 and 0.5 µL/mL and shows inhibitory activity at a concentration of 1 µL/mL and fungicidal activity at concentrations of 1, 1.25 and 2 µL/mL with respective inhibition percentages of 27. 55, 32.37, 68.83, 100, 100 and 10.59, 15.22, 69.94, 100, 100 % at different methods. C. sinensis essential oil has a significantly positive effect on the parasitic and wood-decaying fungus G. resinaceum due to its high content of more than 50% monoterpenes, which are volatile compounds with antibacterial, antiviral and antifungal properties. Conclusion: Essential oils are most often used against microscopic pathogenic fungi in food, the environment and crops, particularly Aspergillus, Fusarium, Candida, Phytophthora and Pythium. This study shows that they can also be applied in the same way as natural extracts to macroscopic parasitic fungi that cause several destructions in fruit trees such as mango and oil palm. 1 30 2026 518 525 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2025.14613 https://phytopharmajournal.com/articles/abstracts/870/year=2025&vol=14&issue=6