none 10.31254/phyto.2025.14606 BioMed Research Publishers BioMed Research Publishers 16195 189925510 316921 20260210015631888 10.31254 2026-02-10T01:56:41Z 2026-02-10T01:56:37Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 1 30 2026 14 6 Wijesooriya A.D.J Thanishka Sandarage M.K Premachandra Tennakoon M.S Anjali Tennakoon https://orcid.org/0009-0005-3909-5726 Shehara L.A Gunawardana https://orcid.org/0000-0001-7415-8992 Pavithra M Kumarapperuma https://orcid.org/0009-0009-2967-8521 Background: Herbal teas are widely consumed beverages globally and are known for their diverse therapeutic properties and health benefits. Objectives: This study aimed to evaluate in vitro acid-neutralizing properties of an herbal tea made from the dried leaves of Aegle marmelos, Desmodium triflorum, and Centella asiatica as a potential management option for gastritis. Materials and Methods: Herbal tea bags (T1-T11) were prepared by blending different ratios of the powdered leaves, and each tea bag was steeped at 100°C for 3, 5, and 7 minutes. The acid-neutralizing capacity was measured using Fordtran’s titration method. Infusions showing the highest acid-neutralizing capacity were further tested at varying temperatures (100°C, 80°C, and 60°C) while maintaining a consistent infusion time. The duration of neutralization was assessed using a modified Vatier’s artificial stomach model. The results were compared with green tea and distilled water. Results: The herbal tea infusions made at 100°C for 5 minutes exhibited significantly higher acid-neutralizing capacities (p ≤ 0.05). The strongest neutralizing capacities were recorded for T9 (27.10 ± 0.00 mL) and T7 (24.70 ± 0.00 mL), followed by green tea (5.43 ± 0.05 mL) and distilled water (0.73 ± 0.05 mL). T9 also showed the longest neutralization duration, lasting 382.33 ± 2.00 seconds, while green tea and distilled water neutralized for only 42.00 ± 1.00 seconds and 72 ± 1.00 seconds, respectively. Conclusion: In conclusion, the T9 formulation, containing only A. marmelos, demonstrated the highest acid-neutralizing ability and duration, making it an optimal solution for managing gastritis when steeped at 100°C for 5 minutes. 1 30 2026 456 462 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2025.14606 https://phytopharmajournal.com/articles/abstracts/863/year=2025&vol=14&issue=6