none 10.31254/phyto.2025.14605 BioMed Research Publishers BioMed Research Publishers 16195 189925509 316921 20260210015631888 10.31254 2026-02-10T01:56:40Z 2026-02-10T01:56:37Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 1 30 2026 14 6 Pragya Shrivastava https://orcid.org/0009-0006-4391-7611 Kush Kumar Nayak https://orcid.org/0000-0003-4000-0323 Zenu Jha Varaprasad Kolla https://orcid.org/0000-0002-6899-6153 Background: Medicinal plants are an important source of bioactive compounds vital for pharmaceutical, cosmetic, and nutraceutical applications. In particular, traditional herbal remedies especially in India utilize leaves of plant, extracts containing a wide variety of secondary metabolites as flavonoids, alkaloids, fatty acids that differ among species. Analytical methods as Gas Chromatography‐Mass Spectrometry (GC‐MS) are widely employed to detect and quantify these metabolites in plant extracts. Objective: The aim of this study was to profile the bioactive constituents present in the acetone extract of leaf of Andrographis paniculata plant by using GC‐MS, and to assess their potential therapeutic properties for possible pharmaceutical applications. Materials and Methods: The leaf plants of A. paniculata were collected and subjected to extraction using acetone as the solvent. Analysis was performed with an Agilent Technologies Mass Hunter GC/MS system, using version B.07.05.2479 for acquisition. The obtained chromatograms were compared with standard reference libraries to identify component compounds are secondary metabolites. Results: Several phytochemicals were detected including piperine, octadecanoic acid (stearic acid), and hexadecenoic acid (palmitic acid). These compounds are known to possess diverse biological activities. Piperine is used antimicrobial, possible anticancer adjuvant. Octadecanoic acid (stearic acid) and hexadecanoic acid (palmitic acid): associated with antioxidant, anti‐inflammatory, antimicrobial or metabolic effects in various studies. The presence of these metabolites suggests A. paniculata acetone extract is rich in bioactive secondary metabolites with multiple therapeutic potentials. Conclusion: The GC–MS analysis of A. paniculata leaf acetone extract revealed bioactive compounds like piperine, stearic acid, and palmitic acid, indicating strong antioxidant, antimicrobial, and anti-inflammatory potential. These findings validate the plant’s traditional medicinal use and suggest its promise as a natural source for developing therapeutic drugs. 1 30 2026 448 455 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2025.14605 https://phytopharmajournal.com/articles/abstracts/862/year=2025&vol=14&issue=6