none 10.31254/phyto.2025.14507 BioMed Research Publishers BioMed Research Publishers 16195 187186658 316921 20251216133144430 10.31254 2025-12-16T13:31:49Z 2025-12-16T13:31:46Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://www.phytopharmajournal.com/ 11 30 2025 14 5 Ankit R. Patel https://orcid.org/0000-0003-0996-9435 Jatin H. Patel Falguni D. Modi Dharmesh R. Patel Ankita D. Verma Background: Escherichia coli (E. coli) infection leads to substantial economic losses in the global poultry industry and the growing issue of antibiotic resistance poses a serious threat to human health. The emergence of antimicrobial resistance due to excessive antibiotic use in poultry production has intensified the search for safer, natural alternatives to control bacterial infections. Objective: This study was conducted to evaluate the hematobiochemical safety profile of a fermented polyherbal formulation in broiler chickens. Materials and Methods: A total of 60 one day old Vencobb 400 broiler chicks were randomly divided into two groups, each comprising 30 birds. Group 1 served as the environmental control and was fed a basal diet without any additional treatment. Group 2 served as test group and received fermented polyherbal extract in drinking water @100 ml/litre. The study was carried out over a period of 42 days. Results: Fermented polyherbal extract demonstrated notable in vitro efficacy against E. coli, with an MIC of 12.5%. Statistical comparisons revealed no significant differences (p > 0.05) between G2 and G1 across all measured parameters: Haemoglobin (g/dl), Total Erythrocyte Count (TEC ×10⁶), Total Leucocyte Count (TLC ×10³), differential white blood cell counts (Heterophils %, Lymphocytes %, Monocytes %, Eosinophils %, Basophils %) and serum biochemical parameters: aspartate transaminase (U/L), alanine transaminase (U/L), total protein (g/dL), uric acid (mg/dL) and albumin (g/dL). All values remained consistent with the control group, suggesting a good safety margin from a hematobiochemical perspective. Conclusion: Findings from this 42-day study indicated that the fermented polyherbal extract is not associated with any observable hepatotoxic or nephrotoxic effects in boiler chickens. Based on the comprehensive analysis of key haematobiochemical parameters, the administration of the fermented polyherbal extract to the test group (G2) for 42 days demonstrated a favorable safety profile relative to the environmental control group (G1). 11 30 2025 346 351 10.31254/phyto.2025.14507 https://phytopharmajournal.com/articles/abstracts/849/year=2025&vol=14&issue=5