none 10.31254/phyto.2025.14501 BioMed Research Publishers BioMed Research Publishers 16195 187186472 316921 20251216133144430 10.31254 2025-12-16T13:31:46Z 2025-12-16T13:31:46Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://www.phytopharmajournal.com/ 11 30 2025 14 5 Pawan Kumar Sagar https://orcid.org/0009-0007-8695-9958 S. Kashyap A.S Khan R.P. Meena K.A. Hafiz Background: Drug standardization research, Physicochemical, HPTLC fingerprinting, quality, safety studies, QC and QA remain a big challenging task for ASU (Ayurvedic, Shiddha, and Unani) formulated drugs. There needs to be more than the QC, QA, and PV scientific screening parameters to validate, authenticate and differentiate hazardous and toxic substances, adulterants in formulated ASU polyherbal drugs. Habb-e-Pachlona is one of the polyherbal Unani classical formulated drug used to treat various human ailments. Objective: This study aims to evaluate the DSR, HPTLC fingerprint, Safety, QC and QA profiling studies of the HEP. The quality, safety and toxicity activities of the HEP and extracts of HEP were also studied. Material and Methods: The physicochemical, phytochemical, and drug-standardization research, along with safety studies such as heavy metal, aflatoxin, and pesticide residue analyses of the coarse powder of the formulated polyherbal HEP pill, were carried out using standard methods. HPTLC of the extracts of HEP were performed using standard methods. The quality and safety effects of the tested HEP drug samples of different microbes, hazardous metaxies and toxins were also investigated. Results: The physicochemical and DSR, HPTLC fingerprint, toxicity, and QC and QA properties of HEP have shown that all the parameters were within the permissible limits. The HPTLC fingerprint profile of the various extracts of HEP exhibited various peaks under UV 254 nm, under UV 366 nm, and under the V-S reagent, visible light region. The HPTLC fingerprint and GC-MS analysis confirmed and illustrated the presence of various bioactive compounds and hazardous adulteration in the test sample. The tested HEP drug samples showed significant quality, safety and toxicity studies against certain pathogenic organisms and promising antimicrobial load activity. Conclusion: The drug standardization research and quality control findings revealed that the test HEP drug was free from adulterations. The physicochemical constants and HPTLC profiles of HEP can be essential data in the future. This polyherbal classical Unani-formulated drug may potent, treat and cures in poor digestion, gastric debility, dyspepsia, anorexia, flatulence, and hiccough/hiccup diseases. 11 30 2025 298 308 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2025.14501 https://phytopharmajournal.com/articles/abstracts/839/year=2025&vol=14&issue=5