none 10.31254/phyto.2025.14409 BioMed Research Publishers BioMed Research Publishers 16195 184331105 316921 20251025020154379 10.31254 2025-10-25T02:02:01Z 2025-10-25T02:01:57Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 09 30 2025 14 4 Anupsingh Vijaysingh Thakur Sonu Ambwani https://orcid.org/0000-0002-6202-3115 Sudhir Kumar https://orcid.org/0009-0002-8236-9364 Tanuj Kumar Ambwani https://orcid.org/0000-0001-6871-135X Background: The growing interest in phytogenic immunomodulators is driven by the need for natural alternatives to synthetic drugs, particularly in animal health. However, many herbal products face skepticism due to limited scientific validation. Clitoria ternatea L., commonly known as butterfly pea, is a traditional medicinal plant rich in diverse phytochemicals such as flavonoids, anthocyanins, alkaloids, and tannins. These compounds are known for their antioxidant, anti-inflammatory, and neuroprotective activities. Traditionally, various parts of the plant- roots, leaves, flowers, and seeds, have been used to treat ailments like fever, wounds, memory disorders, and microbial infections. Objective: The present study was conducted to evaluate the immunostimulatory effects of a 50% hydro-methanolic leaf extract of Clitoria ternatea (CTE) on chicken lymphocytes in vitro, and to validate its potential as a natural immunomodulator for poultry health management. Materials and Methods: Lymphocytes were isolated from healthy broiler chickens under aseptic conditions and cultured in vitro. The cytotoxicity of various concentrations of the CTE was assessed using the MTT assay to determine the maximum non-cytotoxic dose (MNCD). Subsequently, the lymphocyte proliferation assay (LPA) was performed to evaluate immunostimulatory activity. Results: The MNCD of CTE was found to be 0.3 mg/mL, and this concentration was used for further immunological testing in the presence of mitogenic stimulation. CTE at 0.3 mg/mL significantly enhanced lymphocyte proliferation compared to untreated control groups, indicating strong immunostimulatory activity. The results confirm the bioactivity of the extract in modulating immune cell function in vitro. Conclusion: The study demonstrates that Clitoria ternatea leaf extract possesses promising immunopotentiating effects on chicken lymphocytes. These findings support its potential use as a natural immune booster in poultry, and contribute to the scientific validation of Clitoria ternatea as a traditional medicinal herb with valuable veterinary immunopharmacological applications. 09 30 2025 287 290 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2025.14409 https://phytopharmajournal.com/articles/abstracts/837/year=2025&vol=14&issue=4