none 10.31254/phyto.2025.14402 BioMed Research Publishers BioMed Research Publishers 16195 184331098 316921 20251025020154379 10.31254 2025-10-25T02:01:58Z 2025-10-25T02:01:57Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 09 30 2025 14 4 G. Dayanand Reddy https://orcid.org/0000-0001-5907-0749 P Sathiyarajeswaran R Ganesan K Venkatraman T Varalakshmi R Rohinkumar S Sevanthi Arshad Ali A. Jaya Prasanna Kumar Background: Nathaichoori Chooranam (NC), a Siddha formulation, is traditionally used in South India for hyperlipidemia, obesity and digestive disorders. NC, which is rich in phytochemicals such as alkaloids and flavonoids, shows promise in managing metabolic conditions. However, chronic toxicity studies are essential to confirm long-term safety, as long-term use of herbal medicines may lead to adverse effects. Objective: This study evaluates the safety profile of NC in a chronic toxicity model to support its therapeutic application. Materials and Methods: A 90-day chronic toxicity study was conducted using Wistar rats following OECD Guideline 452. Animals were randomized into 6 groups (n=10/sex/group): control (0.5% CMC vehicle) and dose groups (low, intermediate, high; 300, 600, 1000 mg/kg body weight, respectively) of Nathaichoori powder administered orally daily. The parameters assessed included body weight, food intake, hematological and biochemical markers (liver, kidney function), organ weights and histopathology of vital organs (liver, kidney, heart, spleen). Clinical signs and mortality were monitored daily. Results: No mortality or significant clinical abnormalities were observed in the groups. Body weight and food intake were comparable to controls. Hematological parameters (RBC, WBC, hemoglobin) and biochemical markers (ALT, creatinine, urea) did not show dose-related changes. Organ weights were unchanged, and histopathological analysis did not reveal treatment-related lesions in the liver, kidneys or other organs, indicating no chronic toxicity at the doses tested. Conclusion: Nathaichoori Chooranam did not exhibit chronic toxicity in rats at doses up to 1000 mg/kg for 90 days, indicating its safety for long-term use. These findings support the potential of NC as a safe herbal remedy, but human studies are needed to confirm safety and establish therapeutic doses. 09 30 2025 215 222 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2025.14402 https://phytopharmajournal.com/articles/abstracts/830/year=2025&vol=14&issue=4