none 10.31254/phyto.2025.14303 BioMed Research Publishers BioMed Research Publishers 16195 182218940 316921 20250913111828153 10.31254 2025-09-13T11:18:35Z 2025-09-13T11:18:34Z 2 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 07 31 2025 14 3 Sonam Tamchos https://orcid.org/0000-0002-5187-7318 Talat Anjum https://orcid.org/0000-0002-3922-0444 Jaffer Hussain https://orcid.org/0000-0002-1895-6874 Background: Hippophae L. belongs to the family Elaeagnaceae. It is a thorny, deciduous, hardy shrub/small tree wildly growing varied conditions. According to the available literatures, it is widely used in traditional medicinal systems. The plant comprises of pharmacological activities including anti-atherogenic, anti-cancer, anti-microbial, anti-stress, cytoprotective, hepatoprotective, immunomodulatory, radioprotective, and tissue regeneration. Objective: The focus of the study was to determine the pharmacognostic characters and physicochemical analysis of the stem and leaf of Hippophae rhamnoides subsp. turkestanica Rousi. Materials and Methods: The plant parts, i.e., stem and leaves, were collected from Skurbuchan, located at 34° 26' 07" N and 76° 42' 38" E in the Union Territory of Ladakh, India. The macroscopic features were studied under stereomicroscope whereas, the microscopic observations and powder microscopy were carried out using trinocular microscope under different magnifications. The physicochemical properties such as LoD, Ash value, water and alcohol soluble extractive value, and Acid insoluble ash were determined as prescribed in the Ayurvedic Pharmacopoeia of India. Results: The study reveals that the transverse section (T.S.) of the stem shows the xylem as the dominant tissue, with laticiferous canals and fiber patches in the cortical area. The leaf's epidermis consists of tangentially elongated cells with a thin cuticle and stellate hairs on both surfaces. The midrib contains phloem with laticiferous cells and a xylem arranged in a semicircular pattern. The Powder microscopy also supports the features, including palmate and stellate hairs, glandular trichomes, vessels with thickening, and rhomboidal calcium oxalate crystals. The results of the physicochemical parameters were recorded in % w/w and the lowest is reported in the stem and maximum in the case of leaf. The value of loss on drying is more or less similar (0.03% & 0.06), whereas the alcohol and water extractive values are recorded higher in leaf than in the stem. Total ash content in stem and leaf is 1.25% and 1.5%, and the acid-insoluble ash value is also comparatively higher in leaf. Conclusion: The study will help to identify and authenticate crude drug/raw materials or those in powder form, even when added to a formulation as ingredients from adulteration, and also form a base for future studies on formulations. 07 31 2025 141 148 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2025.14303 https://phytopharmajournal.com/articles/abstracts/816/year=2025&vol=14&issue=3