none 10.31254/phyto.2024.13607 BioMed Research Publishers BioMed Research Publishers 16195 170189970 316921 20250113024539019 10.31254 2025-01-13T02:45:52Z 2025-01-13T02:45:43Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://www.phytopharmajournal.com/ 12 31 2024 13 6 Salma Osman Mohamedkhair Osman Lata Paliwal Sonu Ambwani https://orcid.org/0000-0002-6202-3115 Tanuj Kumar Ambwani Indigenous knowledge holds valuable insights into medicinal plants and their traditional uses. Incorporating products derived from medicinal plants as natural antioxidants could play a crucial role in enhancing immunity and overall health. This study investigates the immunomodulatory effects of two plant hydromethanolic extracts viz., Albizia lebbeck (ALE) and Lawsonia inermis (LIE), utilizing a chicken lymphocytes culture model. Both plants were extracted using a hydromethanolic method, revealing significant phytochemical constituents, including flavonoids and phenolics. The percent yield of LIE (6.08%) was higher than that of ALE (5.44%). Phytochemical analysis indicated that ALE contained 124 µg rutin equivalents (RE)/mg of extract for flavonoids and 3.93 µg gallic acid equivalents (GAE)/mg for phenolics, while LIE showed 104.5 µg RE/mg and 1.84 µg GAE/mg, respectively. The maximum non-cytotoxic dose (MNCD) was established at 0.4 mg/ml for ALE and 0.2 mg/ml for LIE, beyond which both the extracts exhibited dose-dependent cytotoxicity. Both extracts enhanced T and B cell proliferation, significantly. These findings suggest that ALE and LIE possess significant immunomodulatory properties, potentially offering therapeutic applications in managing immunity-related conditions. Further research is warranted to elucidate their mechanisms of action and establish optimal dosages for clinical use. 12 31 2024 464 469 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2024.13607 https://www.phytopharmajournal.com/assets/pdf_files/Vol13_Issue6_07.pdf