none 10.31254/phyto.2024.13405 BioMed Research Publishers BioMed Research Publishers 16195 164950409 316921 20240922112415285 10.31254 2024-09-22T11:24:24Z 2024-09-22T11:24:21Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://www.phytopharmajournal.com/ 8 31 2024 13 4 Sreelakshmi Aravind Kuppadakath Varuna Purushothama Panicker Chinnu Mundakkal Vijayan Uma Radhakrishnan Nimna Ajay Athira Narayanan Pepsin soluble collagen (PSC) is a type-1 collagen which is abundant in skin. The pepsin soluble collagen was successfully extracted from the skin of Daggertooth pike conger (Muraenesox cinereus) by using the conventional method of salt precipitation followed by dialysis. Pepsin-soluble collagen showed a yield of 10.12% on wet matter basis and 25.5% on dry matter basis. The triple helical structure of collagen was confirmed by Fourier Transform Infrared Spectroscopy (FTIR) and UV-Visible spectrophotometry. Isolated PSC was confirmed as type 1 collagen by Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). FTIR spectra of PSC suggested that the secondary structure of the triple helical collagen was intact even after pepsin digestion. Pepsin-soluble collagen was soluble in acidic pH with maximum solubility at pH 4. The solubility was found to be decreased with an increase in the concentration of sodium chloride with a minimum solubility at 4%. The present PSC isolate from eel fish exhibited comparatively high thermal stability of 36 ͦ C. Morphological analysis by Scanning electron microscopy (SEM) revealed a porous structure and comparatively high thermal stability makes it a promising biomaterial. 8 31 2024 298 306 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2024.13405 https://www.phytopharmajournal.com/assets/pdf_files/Vol13_Issue4_05.pdf