none 10.31254/phyto.2024.13401 BioMed Research Publishers BioMed Research Publishers 16195 164950405 316921 20240922112415285 10.31254 2024-09-22T11:24:22Z 2024-09-22T11:24:21Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://www.phytopharmajournal.com/ 8 31 2024 13 4 GC Dhaije SR Rajurkar Nitin D Jadhav AR Pawar BW Narladkar GM Chigure MD Jadhav PR Rathod Anil Kumar Sharma The application of different nanoparticles is underway in the development of phyto-medicines. Silver nanoparticles (AgNPs) are commonly used as coating agents to treat various bacterial and viral diseases. Their safety and potential toxicity necessitate further research to explore the development of nanoparticles coated herbal drugs. The Azadirachta indica (neem) 5% leaf extract and silver nitrate was used to prepare the silver nanoparticles (SNPs) with the biological reduction (green synthesis) method [1]. The application of silver nanoparticles (SNPs) coated with J. carcus leaf extract (JcLE) on Wistar rats showed no adverse sign of toxicity on hematological parameters except an increase in the neutrophil count and increased in total erythrocyte count (TEC) on 28th day. Similarly, serum biochemical values exhibited an increase in aspartate aminotransferase (AST/SGOT) and alanine transaminase (ALT/SGPT) with JcLE-coated SNPs in treated groups. The histopathology revealed that there was no alteration in skin architecture, however, minimal alteration was observed in the liver and kidney with dilatation of the central vein and sinusoidal space, fatty change in the liver, multifocal necrobiotic changes, cystic degeneration and mild focal glomerular atrophy and hyaline cast in the lumen of tubules of kidney. It indicates the proper absorption and excretion of SNPs from the body. There was no significant effect on hematological parameters in the rats treated with J. curcas leaf extract-coated SNP. However, the serum biochemical analysis revealed a toxic effect on the kidney. 8 31 2024 275 279 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2024.13401 https://www.phytopharmajournal.com/assets/pdf_files/Vol13_Issue4_01.pdf