none 10.31254/phyto.2024.13302 BioMed Research Publishers BioMed Research Publishers 16195 161288724 316921 20240601223927842 10.31254 2024-06-02T02:39:36Z 2024-06-02T02:39:32Z 2 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://www.phytopharmajournal.com/ 06 01 2024 13 3 Nadine Florence Ambomo Raceline Kamkumo Gounoue Jean Hubert Donfack Florence Tsofack Ngueguim Angèle Carine Bidja Paul Desiré Djomeni Dzeufiet Theophile Dimo Background: Poor nutritional status disrupts bone metabolism leading to fractures. This study investigated the effect of the aqueous leaf extract of Alstonia boonei on fractured rats fed a diet supplemented with oxidised palm oil and sucrose. Methods: The iodine, peroxide and acidity indexes of the oxidised palm oil were determined. Bone damage was induced by feeding rats an oxidised palm oil/sucrose enriched diet for 16 weeks followed by fracture. Fractured animals received distilled water or plant extract (50, 100 or 200 mg/kg) simultaneously with their diet for two weeks. Biochemical parameters including lipid profile, bone markers, oxidative stress and hematological parameters were assessed. X-rays and histology of the femur were performed. Results: Oxidised palm oil showed a significant increase in the peroxide and acidity indexes, while the iodine index decreased. Fractured rats fed a diet enriched with oxidised palm oil and sucrose exhibited dyslipidemia, altered haematological parameters and oxidative stress bone ALP activity with poor natural bone formation. Fracture induced a decrease in bone calcium and an increase in bone ALP. A. boonei extract improved lipid profile, hematological and oxidative stress parameters. There was a significant increase in femoral calcium and a significant decrease in femoral ALP. In addition, a better organisation of the bone architecture was observed, with a marked effect at a dose of 100 mg/kg. Conclusion: These results suggest that A. boonei leaf improves bone repair in poor nutritional conditions, thanks to its hypolipidemic and antioxidant properties mediated by the minerals and secondary metabolites contained in the extract. 06 01 2024 197 207 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2024.13302 https://www.phytopharmajournal.com/assets/pdf_files/Vol13_Issue2_02.pdf