none 10.31254/phyto.2024.13207 BioMed Research Publishers BioMed Research Publishers 16195 160621357 316921 20240511112941908 10.31254 2024-05-11T15:29:52Z 2024-05-11T15:29:46Z 3 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://www.phytopharmajournal.com/ 4 30 2024 13 2 Jaysing Mahavirsing Dinore https://orcid.org/0000-0002-0819-4984 Harshal Shivaji Patil https://orcid.org/0000-0002-6388-8663 Samreen Fatema Farooqui https://orcid.org/0000-0002-7721-8089 Krishna Raju Nagare Jalindar Sakharam Ambhore Mazahar Farooqui https://orcid.org/0000-0003-2236-6639 Cajanus cajan, a well-known legume crop with nutritional significance, has been used traditionally for various therapeutic purposes. Despite its ethnomedicinal importance and rich metabolite composition, there have been limited investigations into the secondary metabolites present in its stems using advanced analytical techniques. This study aimed to enhance our understanding of the chemical composition and biological activities of underutilized C. cajan stems. GC-MS & LC-MS analysis revealed flavonoids as the stem's predominant secondary metabolites. The methanol extract exhibited potent antibacterial activity against E. coli, S. aureus, and C. albicans. Major bioactive compounds, including rutin, apiin, quercetrin, and aesculine, contributed to these beneficial effects. The study also revealed a high total phenolic content of 121.5 mg GAE/g in the stem extract, further establishing C. cajan stems as a valuable source of therapeutic molecules. 4 30 2024 133 142 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2024.13207 https://www.phytopharmajournal.com/assets/pdf_files/Vol13_Issue2_07.pdf