none 10.31254/phyto.2024.13102 BioMed Research Publishers BioMed Research Publishers 16195 159494100 316921 20240402201034073 10.31254 2024-04-03T00:10:41Z 2024-04-03T00:10:38Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 2 29 2024 13 1 Ganesh Pawar http://orcid.org/0000-0002-4505-9845 Samiksha Parihar http://orcid.org/0000-0003-3098-2618 Hari Om Saxena http://orcid.org/0000-0001-7239-9880 Holarrhena antidysenterica is an important medicinal plant in the Ayurvedic system of medicine. The present study aimed to screen secondary metabolites qualitatively and quantitively and to develop chemical fingerprint profiles of stem bark of the target species. The phytochemical screening findings revealed that the methanolic extract of stem bark contained alkaloids, flavonoids, phenols, steroids, tannins, cardiac glycosides, and terpenoids. HPTLC fingerprint profiles were developed which were specific to the mobile phase Toluene: Ethyl acetate: Diethylamine (6.5:2.5:1 v/v/v) and Rf values. The fingerprints are a valuable tool for quality assurance since these can be used as biochemical markers to differentiate between authentic drugs and adulterants. 2 29 2024 4 11 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2024.13102 https://phytopharmajournal.com/assets/pdf_files/Vol13_Issue1_02.pdf