none 10.31254/phyto.2024.13101 BioMed Research Publishers BioMed Research Publishers 16195 159494098 316921 20240402201034073 10.31254 2024-04-03T00:10:40Z 2024-04-03T00:10:38Z 1 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://phytopharmajournal.com/ 2 29 2024 13 1 Alfin Mariya Nisaath Begum Arya Mohan S Sujith AR Nisha Phytochemicals and their derivatives are promising options for improving cancer treatment efficiency while minimising side effects. Methanolic extract of flowers of Mallotus philippensis were assessed for their cytotoxicity in MCF-7cell line by 3-(4,5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay at concentrations of 640, 320, 160, 80, 40, 20, 10 and 5 µg/mL and the half maximal inhibitory concentration (IC50) was calculated using Graph Pad Prism 5.0. As a positive control, doxorubicin was used. Phytochemical analysis of M. philippensis methanolic extract using standard tests revealed the presence of alkaloids, tannins, flavonoids, and phenolic compounds. When the cells were exposed to different concentrations of the extracts, a dose-dependent reduction in cell viability was observed. The IC50 of M. philippensis methanolic extract is 41.28 µg/mL. The antiproliferative effect of methanolic extract of M. philippensis in cancer cells in a dose dependent manner could be a promising strategy in chemotherapy. This extrapolates the use of natural products in drug designing conducting clinical trials in vivo and in human subjects. 2 29 2024 1 3 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2024.13101 https://phytopharmajournal.com/assets/pdf_files/Vol13_Issue1_01.pdf