none 10.31254/phyto.2023.12505 BioMed Research Publishers BioMed Research Publishers 16195 155559628 316921 20231129193611849 10.31254 2023-11-30T00:36:22Z 2023-11-30T00:36:16Z 1 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto https://www.phytopharmajournal.com/ 10 30 2023 12 5 Salma Fathima Pruthvi Charan Jambiga Ramya Thumma Samreen Ahmadi Srinitha Askani Bushra Sumayya Mohammed Poornesh Sutramay Sunitha Bai Dharavath Shasthree Taduri Background: Andrographis paniculata is a medicinal plant with a long history of traditional use in various herbal medicine systems. Objective: This study aims to conduct aphytochemical screening and antibacterial activity from leaf, stem and root parts of Andrographis paniculata. Methods: The preliminary screening was done by cold maceration technique using chloroform, butanol, methanol, ethanol and aqueous solvents. This phytochemical screening showed the presence of alkaloids, glycosides, flavonoids, terpenoids, phenols, tannins and saponins. Antibacterial activity was performed by using well diffusion method from leaf, stem and root butanolic extracts of A. paniculata. Results: Glycosides are abundantly present in leaf, stem and root explant extracts. Whereas, alkaloids and flavonoids are moderately present in leaf, stem and root explant extracts and followed by less presence of tannins, phenols and saponins. The maximum zone of bacterial inhibition was observed in leaf butanolic extracts against of the E.Coli, for stem butanolic extract against of B. subtilis and for root butanolic extract against B. sphaericus. Conclusion: This study revealed the potential photochemical constituents and antibacterial activity in A.Paniculata. The presence of these bioactive substances they use in treatment. 10 30 2023 305 310 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/phyto.2023.12505 https://phytopharmajournal.com/assets/pdf_files/Vol12_Issue5_05.pdf