none 10.31254/phyto.2021.10301 BioMed Research Publishers BioMed Research Publishers 16195 130429445 316921 20220826003753959 10.31254 2022-08-26T04:37:57Z 2021-09-23T15:44:19Z 0 The Journal of Phytopharmacology J Phytopharmacol 2320480X 10.31254/phyto http://www.phytopharmajournal.com/ 05 24 2021 10 3 Jamila Saleh Funsho Olowoniyi Ekpa Emmanuel Abdulrahman Abdullateef Muhibat Komolafe Bolanle Madinat Hassan Throughout the history of man, traditional and herbal method of treatment of diseases has been used without considering the dose effect. Therefore, this present study is an attempt on investigating the effect of different doses of Annona squamosa methanolic leaf extract on male wistar Rats especially the delicate organs. The work involves oral administration of different doses (10, 100, 1000, 1600, 2900, 5000 mg/kg body weight) of the extract to groups of rats according to Lorkes method. The animals were monitored for 30 days at every 24 hours interval in order to find the median lethal dose (LD50) of the extract. Internal organ-body weight ratios (OBR) of animals in the test groups were determined and compared with those of the control group. LD50 was found to be greater than 5000mg/kg body weight without any significant decrease (p>0.05) in body weight. Biochemical analysis of Aspartate amino transferase (AST), Alanine amino transferase (ALT), Albumin and globulin of animals administered with extract showed no significant difference compared to the control groups (p>0.05) but concentration of Alkaline Phosphatase (ALP) indicated obvious changes in the treated groups compared to the control groups (p<0.05). Histopathology of the kidney revealed some inflammation at 1000, 1600, and 5000 mg/kg body weight. The implications of using this extract within safe doses in traditional medicine is hereby discussed. 05 24 2021 151 155 1 10.31254/BIOMED-CROSSMARK-POLICY www.biomedresearch.org false 10.31254/phyto.2021.10301 http://www.phytopharmajournal.com/Vol10_Issue3_01.pdf