none 10.31254/dentistry.2025.10306 BioMed Research Publishers BioMed Research Publishers 16195 191232955 314763 20260308052548514 10.31254 2026-03-08T05:25:55Z 2026-03-08T05:25:52Z 0 International Journal of Dentistry Research Int J Dentistry Res 25813218 10.31254/dentistry https://www.dentistryscience.com/ 01 17 2026 10 3 Manav Varshney Post Graduate, Department of Periodontology and Oral Implantology, ITS Centre for Dental Studies and Research, Ghaziabad -201206, Uttar Pradesh, India https://orcid.org/0009-0004-7651-8313 Akash A Bora Post Graduate, Department of Periodontology and Oral Implantology, ITS Centre for Dental Studies and Research, Ghaziabad -201206, Uttar Pradesh, India https://orcid.org/0009-0000-5583-1176 Rahul Chopra Professor, Department of Periodontology and Oral Implantology, ITS Centre for Dental Studies and Research, Ghaziabad - 201206, Uttar Pradesh, India https://orcid.org/0000-0003-1279-1666 Sumit Malhotra Professor and Head, Department of Periodontology and Oral, Implantology, ITS Centre for Dental Studies and Research, Ghaziabad-201206, Uttar Pradesh, India https://orcid.org/0000-0002-4257-8762 Background: Chronic periodontitis is a common inflammatory disease leading to the destruction of periodontal tissues. While scaling and root planing (SRP) remains the cornerstone of treatment, adjunctive agents can enhance therapeutic outcomes. Propolis, a natural bioactive compound, possesses significant antimicrobial and anesthetic properties. This split-mouth study was conducted to evaluate the efficacy of 10% Propolis hydrogel as an adjunct to SRP in improving clinical parameters and reducing microbial load in generalized chronic periodontitis. Methodology: 15 patients with generalized chronic periodontitis having pocket depth ≥ 4mm were selected. Maxillary and mandibular arch of each patient was randomly divided to receive either of Placebo gel (Group A) or 10% Propolis Hydrogel (Group B) followed by SRP and application of gel in periodontal pockets. Clinical parameters, VAS score were assessed and quantitative analysis of sub-gingival plaque samples for aerobic and anaerobic bacterial growths using colony count method at baseline and after 3 months was performed. Results: Intragroup comparison shows statistically significant results in all the clinical and microbiological parameters. Intergroup comparison showed statistically significant amount of reduction in pockets and Colony Forming Unit (CFU/ml) of aerobic as well as anaerobic bacteria in Group B when compared to Group A. Significant results were also found in Group B in terms of VAS score (assessing dentinal sensitivity) in both the groups. Conclusion: The adjunctive use of 10% propolis hydrogel with SRP provides enhanced anesthetic and antimicrobial benefits in the treatment of generalized chronic periodontitis, suggesting its potential as a valuable adjunct to mechanical therapy. 01 17 2026 102 109 1 10.31254/biomed-crossmark-policy www.biomedresearch.org false 10.31254/dentistry.2025.10306 https://www.dentistryscience.com/IJDR_2025103_06.pdf