none 10.1186/s13104-022-05982-9 Springer Science and Business Media LLC Springer (Biomed Central Ltd.) 297 135385829 76986 20220310120527228 10.1186 2022-03-10T11:07:41Z 2022-03-10T11:05:59Z 0 BMC Research Notes BMC Res Notes 1756-0500 12 2022 15 1 Jyotshana Gautam Hui Xu Junxi Hu Christa Pennacchio Anna Lipzen Joel Martin Zhaohui Xu http://orcid.org/0000-0002-1866-3254 Abstract Objective Adaptive laboratory evolution (ALE) is an effective approach to study the evolution behavior of bacterial cultures and to select for strains with desired metabolic features. In this study, we explored the possibility of evolving Thermotoga sp. strain RQ7 for cellulose-degrading abilities. Results Wild type RQ7 strain was subject to a series of transfers over six and half years with cellulose filter paper as the main and eventually the sole carbon source. Each transfer was accompanied with the addition of 50 μg of Caldicellulosiruptor saccharolyticus DSM 8903 genomic DNA. A total of 331 transfers were completed. No cellulose degradation was observed with the RQ7 cultures. Thirty three (33) isolates from six time points were sampled and sequenced. Nineteen (19) of the 33 isolates were unique, and the rest were duplicated clones. None of the isolates acquired C. saccharolyticus DNA, but all accumulated small-scale mutations throughout their genomes. Sequence analyses revealed 35 mutations that were preserved throughout the generations and another 15 mutations emerged near the end of the study. Many of the affected genes participate in phosphate metabolism, substrate transport, stress response, sensory transduction, and gene regulation. 03 10 2022 12 2022 99 5982 1 10.1007/springer_crossmark_policy link.springer.com false 27 September 2021 22 February 2022 10 March 2022 Not applicable. Not applicable. The authors declare that they have no competing interest. 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