none 10.1126/science.1234168 American Association for the Advancement of Science (AAAS) American Association for the Advancement of Science (AAAS) 221 62591062 5530 2024011005352800286 10.1126 2024-01-10T14:50:02Z 2013-06-06T18:13:33Z 185 Science Science 0036-8075 1095-9203 06 07 2013 340 6137 Philipp J. Keller Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA. Morphogenesis, the development of the shape of an organism, is a dynamic process on a multitude of scales, from fast subcellular rearrangements and cell movements to slow structural changes at the whole-organism level. Live-imaging approaches based on light microscopy reveal the intricate dynamics of this process and are thus indispensable for investigating the underlying mechanisms. This Review discusses emerging imaging techniques that can record morphogenesis at temporal scales from seconds to days and at spatial scales from hundreds of nanometers to several millimeters. To unlock their full potential, these methods need to be matched with new computational approaches and physical models that help convert highly complex image data sets into biological insights. 06 07 2013 1234168 10.1126/science.1234168 10.1126/science.1234168 https://www.science.org/doi/10.1126/science.1234168 https://www.science.org/doi/pdf/10.1126/science.1234168 10.1126/science.1167094 10.1126/science.1162493 10.1073/pnas.1108494108 10.1038/nbt.2281 10.1016/j.cell.2008.01.053 10.1016/j.ceb.2010.12.004 10.1016/j.devcel.2011.12.007 10.1126/science.1225399 10.1126/science.1235249 10.1126/science.2321027 10.1111/j.1365-2818.1993.tb03346.x 10.1126/science.1100035 10.1002/andp.19023150102 10.1038/nmeth.1709 10.1002/dvg.20698 10.1038/nmeth.2434 10.1126/science.1195929 10.1038/nmeth.1411 10.1038/nphoton.2012.205 10.1038/nmeth.2098 10.1038/nmeth.1586 10.1016/j.cell.2012.10.008 10.1038/nbt.1928 10.1038/nmeth.1652 10.1038/nmeth.2062 10.1038/nmeth.2064 10.1038/nphoton.2010.204 10.1364/OL.22.001905 10.1529/biophysj.107.120345 10.1038/nmeth.1476 10.1098/rsta.2007.0013 10.1038/nphoton.2010.306 10.1038/nbt1037 10.1152/physrev.00038.2009 10.1038/nature11589 10.1126/science.1221071 10.1126/science.1224143 10.1038/nprot.2009.130 10.1038/nature10938 10.1007/978-1-61779-210-6_9 10.1016/j.gde.2010.05.008 10.1371/journal.pbio.1001256 10.1038/nmeth.1472 10.1073/pnas.0511111103 10.1038/nmeth.1228 10.1038/nbt.1612 10.1038/nmeth.2084 10.1038/nrg2548 10.1038/nature09198 10.1126/science.1189428 10.1242/dev.085001